FRS2 (phospho Y436) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated Huvec cells, Lane 2: HuvEc cells, Lane 3: JK cells with FRS2 (phospho Y436) polyclonal antibody (Cat # PAB29226) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by FRS2 (phospho Y436) polyclonal antibody (Cat # PAB29226) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human FRS2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y436 of human FRS2.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of FRS2 only when phosphorylated at tyrosine 436.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated Huvec cells, Lane 2: HuvEc cells, Lane 3: JK cells with FRS2 (phospho Y436) polyclonal antibody (Cat # PAB29226) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by FRS2 (phospho Y436) polyclonal antibody (Cat # PAB29226) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — FRS2
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Interactome
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Pathway
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Disease
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