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Last updated: 2017/7/23

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FOS (phospho T232) polyclonal antibody

  • Catalog # : PAB29224
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human FOS.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T232 of human FOS.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 48
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody detects endogenous levels of FOS only when phosphorylated at threonine 232.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: antigen-specific peptide treated COS7 cells, Lane 2: COS7 cells with FOS (phospho T232) polyclonal antibody (Cat # PAB29224) at 1:500-1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human breast carcinoma tissue by FOS (phospho T232) polyclonal antibody (Cat # PAB29224) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2353
  • Gene Name:
  • FOS
  • Gene Alias:
  • AP-1,C-FOS
  • Gene Description:
  • v-fos FBJ murine osteosarcoma viral oncogene homolog
  • Gene Summary:
  • The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq
  • Other Designations:
  • FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS),activator protein 1,cellular oncogene c-fos
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