STK39 (phospho S309) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human STK39.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S309 of human STK39.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of STK39 only when phosphorylated at serine 309.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: COLO205 cells Lane 2: antigen-specific peptide treated COLO205 cells with STK39 (phospho S309) polyclonal antibody (Cat # PAB29220).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by STK39 (phospho S309) polyclonal antibody (Cat # PAB29220) without blocking peptide (A) or preincubated with blocking peptide (B). -
Gene Info — STK39
Entrez GeneID
27347Protein Accession#
Q9UEW8Gene Name
STK39
Gene Alias
DCHT, DKFZp686K05124, PASK, SPAK
Gene Description
serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Omim ID
607648Gene Ontology
HyperlinkGene Summary
This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq
Other Designations
Ste20-like protein kinase|proline-alanine-rich STE20-related kinase|small intestine SPAK-like kinase
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