PAK1 (phospho S204) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated 3T3 cells, Lane 2: antigen-specific peptide treated 3T3 cells with PAK1 (phospho S204) polyclonal antibody (Cat # PAB29215) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by PAK1 (phospho S204) polyclonal antibody (Cat # PAB29215) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human PAK1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S204 of human PAK1.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of PAK1 only when phosphorylated at serine 204.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated 3T3 cells, Lane 2: antigen-specific peptide treated 3T3 cells with PAK1 (phospho S204) polyclonal antibody (Cat # PAB29215) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by PAK1 (phospho S204) polyclonal antibody (Cat # PAB29215) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — PAK1
Entrez GeneID
5058Protein Accession#
Q13153Gene Name
PAK1
Gene Alias
MGC130000, MGC130001, PAKalpha
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 1
Omim ID
602590Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
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Interactome
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Pathway
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Disease
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