Western blot analysis of Lane 1: Serum treated Jurkat cells Lane 2: antigen-specific peptide treated Jurkat cells with MAP2K3 (phospho T222) polyclonal antibody (Cat # PAB29210) at 1:500-1000 dilution.
Immunohistochemical staining of human brain tissue by MAP2K3 (phospho T222) polyclonal antibody (Cat # PAB29210) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq