MAP2K3 (phospho T222) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: Serum treated Jurkat cells Lane 2: antigen-specific peptide treated Jurkat cells with MAP2K3 (phospho T222) polyclonal antibody (Cat # PAB29210) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by MAP2K3 (phospho T222) polyclonal antibody (Cat # PAB29210) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MAP2K3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T222 of human MAP2K3.
Host
Rabbit
Theoretical MW (kDa)
39
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Serum treated Jurkat cells Lane 2: antigen-specific peptide treated Jurkat cells with MAP2K3 (phospho T222) polyclonal antibody (Cat # PAB29210) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by MAP2K3 (phospho T222) polyclonal antibody (Cat # PAB29210) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — MAP2K3
Entrez GeneID
5606Protein Accession#
P46734Gene Name
MAP2K3
Gene Alias
MAPKK3, MEK3, MKK3, PRKMK3
Gene Description
mitogen-activated protein kinase kinase 3
Omim ID
602315Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq
Other Designations
MAP kinase kinase 3|MAPK/ERK kinase 3|OTTHUMP00000166044|dual specificity mitogen activated protein kinase kinase 3
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