MAP3K1 (phospho T1400) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells with MAP3K1 (phospho T1400) polyclonal antibody (Cat # PAB29207) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by MAP3K1 (phospho T1400) polyclonal antibody (Cat # PAB29207) without blocking peptide (A) or preincubated with blocking peptide (B).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MAP3K1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T1400 of human MAP3K1.
Host
Rabbit
Theoretical MW (kDa)
130
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells with MAP3K1 (phospho T1400) polyclonal antibody (Cat # PAB29207) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by MAP3K1 (phospho T1400) polyclonal antibody (Cat # PAB29207) without blocking peptide (A) or preincubated with blocking peptide (B). -
Gene Info — MAP3K1
Entrez GeneID
4214Protein Accession#
Q13233Gene Name
MAP3K1
Gene Alias
MAPKKK1, MEKK, MEKK1
Gene Description
mitogen-activated protein kinase kinase kinase 1
Omim ID
600982Gene Ontology
HyperlinkGene Summary
MAP3K, or MEK kinase, is a serine/threonine kinase that occupies a pivotal role in a network of phosphorylating enzymes integrating cellular responses to a number of mitogenic and metabolic stimuli, including insulin (MIM 176730) and many growth factors.[supplied by OMIM
Other Designations
MAP/ERK kinase kinase 1|MAPK/ERK kinase kinase 1|MEK kinase 1|OTTHUMP00000065818
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