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Last updated: 2017/12/17

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MAP3K1 (phospho T1400) polyclonal antibody

  • Catalog # : PAB29207
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MAP3K1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T1400 of human MAP3K1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 130
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells with MAP3K1 (phospho T1400) polyclonal antibody (Cat # PAB29207) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human brain tissue by MAP3K1 (phospho T1400) polyclonal antibody (Cat # PAB29207) without blocking peptide (A) or preincubated with blocking peptide (B).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4214
  • Gene Name:
  • MAP3K1
  • Gene Alias:
  • MAPKKK1,MEKK,MEKK1
  • Gene Description:
  • mitogen-activated protein kinase kinase kinase 1
  • Gene Summary:
  • MAP3K, or MEK kinase, is a serine/threonine kinase that occupies a pivotal role in a network of phosphorylating enzymes integrating cellular responses to a number of mitogenic and metabolic stimuli, including insulin (MIM 176730) and many growth factors.[supplied by OMIM
  • Other Designations:
  • MAP/ERK kinase kinase 1,MAPK/ERK kinase kinase 1,MEK kinase 1,OTTHUMP00000065818
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