Chicken polyclonal antibody raised against c-Myc tag with HRP conjugation.
Asynthetic peptide corresponding to c-Myc tag and coupled to KLH.
Antibody specificity was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL-BSA). A 1:10,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction. An appropriate specificity controls were performed in parallel.
Antigen affinity purification
Western Blot (1:1000) The optimal working dilution should be determined by the end user.