SPP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of cell extracts from MCF-7 cells transfected with Lane 1: Osteopontin-a, Lane 2: Osteopontin-c, Lane 3: vector with SPP1 polyclonal antibody (Cat # PAB29191).
Western Blot (Recombinant protein)
Western blot analysis of GST-tagged Osteopontin-c (Lane 1, Lane 3) and Osteopontin-a (Lane 2, Lane 4) recombinant protein with SPP1 polyclonal antibody (Cat # PAB29191) (Lane 1, Lane2) or reprobed anti-pan-Osteopontin O17 antibody.
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Specification
Product Description
Chicken polyclonal antibody raised against the synthetic peptide of Human Osteopontin-c.
Immunogen
A synthetic peptide corresponding to the splice junction of osteopontin-c.
Sequence
SEEKQNAVSC
Host
Chicken
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgY
Recommend Usage
ELISA (2 ug/ml)
Western Blot (1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of cell extracts from MCF-7 cells transfected with Lane 1: Osteopontin-a, Lane 2: Osteopontin-c, Lane 3: vector with SPP1 polyclonal antibody (Cat # PAB29191).Western Blot (Recombinant protein)
Western blot analysis of GST-tagged Osteopontin-c (Lane 1, Lane 3) and Osteopontin-a (Lane 2, Lane 4) recombinant protein with SPP1 polyclonal antibody (Cat # PAB29191) (Lane 1, Lane2) or reprobed anti-pan-Osteopontin O17 antibody.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPP1
Entrez GeneID
6696Gene Name
SPP1
Gene Alias
BNSP, BSPI, ETA-1, MGC110940, OPN
Gene Description
secreted phosphoprotein 1
Omim ID
166490Gene Ontology
HyperlinkGene Summary
bone sialoprotein I
Other Designations
SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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Interactome
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Pathway
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Disease
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