CDK8 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of CDK8 polyclonal antibody (Cat # PAB2918) in HL-60 cell lysate. CDK8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDK8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CDK8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDK8 polyclonal antibody (Cat # PAB2918) in HL-60 cell lysate. CDK8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. -
Gene Info — CDK8
Entrez GeneID
1024Protein Accession#
P49336Gene Name
CDK8
Gene Alias
K35, MGC126074, MGC126075
Gene Description
cyclin-dependent kinase 8
Omim ID
603184Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq
Other Designations
CDK8 protein kinase|OTTHUMP00000018158|cell division protein kinase 8|protein kinase K35
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Interactome
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Publication Reference
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TFIIH is negatively regulated by cdk8-containing mediator complexes.
Akoulitchev S, Chuikov S, Reinberg D.
Nature 2000 Sep; 407(6800):102.
Application：WB-Tr, Human, HEK 293T cells.
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Genomics of the human genes encoding four TAFII subunits of TFIID, the three subunits of TFIIA, as well as CDK8 and SURB7.
Di Pietro C, Rapisarda A, Bonaiuto C, Lizzio MN, Engel H, Amico V, Scalia M, Amato A, Grzeschik KH, Sichel G, Purrello M.
Somatic Cell and Molecular Genetics 1999 May; 25(3):185.
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Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases.
Rickert P, Corden JL, Lees E.
Oncogene 1999 Jan; 18(4):1093.
Application：KA, Recombinant protein.
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TFIIH is negatively regulated by cdk8-containing mediator complexes.
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