Chicken anti-Rabbit IgG secondary antibody (HRP)
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Specification
Product Description
Chicken polyclonal antibody raised against Rabbit IgG. The antibody is conjugated with HRP.
Immunogen
Purified rabbit IgG (whole protein).
Host
Chicken
Reactivity
Rabbit
Form
Liquid
Conjugation
HRP
Purification
Antigen affinity purification
Isotype
IgY
Quality Control Testing
This antibody was tested for specificity using double- immunodiffusion in agarose gels and ELISA analysis. In the latter, a 1:70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using tetramethylbenzidine as substrate.
Recommend Usage
ELISA
Western Blot(1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM HEPES, pH 7.0, 0.1M NaCl, (0.2% BSA, 0.01% thimerosal).
Storage Instruction
Store at 4°C and avoid from light. Do not freeze unless for long term storage, store at -20°C with 50% glycerol.
Aliquot to avoid repeated freezing and thawing.Note
This product contains thimerosol: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis.
Ji D, Jiang C, Zhang L, Liang N, Jiang T, Yang B, Liang H.
Journal of Cellular Physiology 2018 Sep; [Epub].
Application：WB, Human, HepG2 cells.
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LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis.
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