Goat anti-Chicken IgY secondary antibody (Biotin)
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Specification
Product Description
Goat polyclonal antibody raised against Chicken IgY. The antibody is conjugated with Biotin.
Immunogen
Purified chicken IgY (whole protein) emulsified in Freund's adjuvant.
Host
Goat
Reactivity
Chicken
Form
Liquid
Conjugation
Biotin
Purification
Affinity purification
Quality Control Testing
This antibody was analyzed using immunoelectrophoresis and a sandwich ELISA. In this latter test, a 1:500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with tetramethylbenzidine as the substrate.
Recommend Usage
ELISA
Immunocytochemistry
Immunohistochemistry
Western Blot(1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide)
Storage Instruction
Store at 4°C and avoid from light. For long term storage, dilute the antibody with sterile glycerol and store it at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
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