VIM polyclonal antibody
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More Files
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VIM polyclonal antibody (Cat # PAB29095) was analyzed by immunocytological staining (at a concentration of 3 ug/mL).
VIM staining (green) in cultured neuronal stem cells from e13.5 mouse brain.
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Specification
Product Description
Chicken polyclonal antibody raised against recombinant Human VIM.
Immunogen
Three different KLH-conjugated synthetic peptides corresponding to different regions of the VIM gene product, shared between the human (NP_003371, NCBI) and mouse (NP_035831, NCBI) sequences.
Host
Chicken
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgY
Quality Control Testing
Immunocytochemistry
VIM polyclonal antibody (Cat # PAB29095) was analyzed by immunocytological staining (at a concentration of 3 ug/mL).
VIM staining (green) in cultured neuronal stem cells from e13.5 mouse brain.Recommend Usage
Immunocytochemistry(1:1000-1:2000)
Immunohistochemistry(1:1000-1:2000)
Western Blot(1:2000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide)
Storage Instruction
Store at 4°C and avoid from light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
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Gene Info — VIM
Entrez GeneID
7431Protein Accession#
NP_003371Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Gene Info — Vim
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Interactome
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Disease
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