Chicken polyclonal antibody raised against recombinant Human GAD1.
A KLH-conjugated synthetic peptide corresponding to a region near the C-terminus of GAD1 gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products.
Human, Mouse, Rat
Antigen affinity purification
Quality Control Testing:
The antibody was analyzed by immunocytological staining (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution) as the secondary reagent. A tissue section through an adult mouse brain showing GAD1 (red staining) in basket cells of the hippocampal formation. Green staining is autofluorescence from GFP expressed in this transgenic mouse.
Immunocytochemistry(1:1000-1:2000) Immunohistochemistry(1:1000-1:2000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (0.02% sodium azide)
Store at 4°C and avoid from light. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq