Chicken polyclonal antibody raised against recombinant Human GRM2.
A KLH-conjugated synthetic peptide corresponding to a unique sequence within GRM2 gene product, shared between the human (Q14416.2, NCBI) and mouse (Q14B12.2, NCBI) sequences.
Antigen affinity purification
Quality Control Testing:
GRM2 polyclonal antibody (Cat # PAB29070) was validated by immunocytological staining (at a concentration of 1 ug/mL). GRM2-staining of pyramidal neurons in the hipocampal formation of an adult mouse brain (red). Green staining is GFP-immunoreactivity for a subpopulation of cortical neurons.
Immunocytochemistry(1:1000-1:2000) Immunohistochemistry(1:1000-1:2000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (0.02% sodium azide)
Store at 4°C and avoid from light. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq