Chicken polyclonal antibody raised against recombinant Human GRM1.
Three different KLH-conjugated synthetic peptides corresponding to different regions of GRM1 gene product, shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences.
Antigen affinity purification
Quality Control Testing:
GRM1 polyclonal antibody (Cat # PAB29069) was validated by immunocytological staining (at a concentration of 3 ug/mL). GRM1-staining of cortical neurons in an adult transgenic mouse brain (red staining). Green staining is GFP-immunoreactivity for a subpopulation of cortical neurons.
Immunocytochemistry(1:1000-1:2000) Immunohistochemistry(1:1000-1:2000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (0.02% sodium azide)
Store at 4°C and avoid from light. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The canonical alpha isoform of the metabotropic glutamate receptor 1 gene is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. Alternative splicing results in multiple transcript variants encoding distinct isoforms; some of which may have distinct functions. [provided by RefSeq