c-Myc polyclonal antibody
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Specification
Product Description
Chicken polyclonal antibody raised against recombinant c-Myc peptide.
Immunogen
A KLH-conjugated synthetic peptide corresponding to c-Myc tag .
Sequence
EQKLISEEDL
Host
Chicken
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgY
Quality Control Testing
This antibody was analyzed using ELISA and western blots using an N-terminal c-Myc-tagged protein. Secondary antibodies used in these qualitycontrol assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies, respectivel.
Recommend Usage
ELISA
Immunocytochemistry(1:250)
Immunohistochemistry
Immunoprecipitation(1:250)
Western Blot(1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide)
Storage Instruction
Store at 4°C and avoid from light. For long term storage, dilute the antibody with sterile glycerol and store it at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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