Chicken polyclonal antibody raised against recombinant HA peptide.
A KLH-conjugated synthetic peptide corresponding to HA tag emulsified in Freund's adjuvant.
Antigen affinity purification
Quality Control Testing:
This antibody was analyzed using ELISA and western blot analyses. By ELISA, the antibody was at 1:10,000 dilution and gave an optical density of >1.0 in a 15-minute reaction using tetramethyl benzidine as the substrate against peptide conjugated to a different carrier protein. By western blot, the This antibody was diluted 1,000-fold and gave a prominent band.
Western Blot(1:5000) The optimal working dilution should be determined by the end user.