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Last updated: 2016/11/27

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HA polyclonal antibody (FITC)

  • Catalog # : PAB29058
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  • Specification
  • Product Description:
  • Chicken polyclonal antibody raised against recombinant HA peptide.
  • Immunogen:
  • A KLH-conjugated synthetic peptide corresponding to HA tag emulsified in Freund's adjuvant.
  • Sequence:
  • YPYDVPDYA
  • Host:
  • Chicken
  • Form:
  • Liquid
  • Conjugation:
  • FITC
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgY
  • Quality Control Testing:
  • Unlabeled antibody was varified with ELISA and western blot. ELISA analysis involved plates coated with peptide conjugated to carrier protein and HRP-labeled goat anti-chicken IgY at 1:10,000 dilution. Under these conditions, the unlabelled chicken anti-HA antibodies (1:10,000 dilution) produced an optical density of >1.0 in 15 minutes. In western blot, the unlabeled antibody at 1:1,000 dilution gave a prominent band with HRP-labeled goat anti-chicken IgY at 1:10,000 dilution.
  • Recommend Usage:
  • Immunocytochemistry
    Immunohistochemistry(1:250)
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.2% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C and avoid from light. For long term storage, dilute the antibody with sterile glycerol and store it at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Immunocytochemistry
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • Immunocytochemistry
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