Chicken polyclonal antibody raised against recombinant HA peptide.
A KLH-conjugated synthetic peptide corresponding to HA tag emulsified in Freund's adjuvant.
Antigen affinity purification
Quality Control Testing:
Unlabeled antibody was varified with ELISA and western blot. ELISA analysis involved plates coated with peptide conjugated to carrier protein and HRP-labeled goat anti-chicken IgY at 1:10,000 dilution. Under these conditions, the unlabelled chicken anti-HA antibodies (1:10,000 dilution) produced an optical density of >1.0 in 15 minutes. In western blot, the unlabeled antibody at 1:1,000 dilution gave a prominent band with HRP-labeled goat anti-chicken IgY at 1:10,000 dilution.
Immunocytochemistry Immunohistochemistry(1:250) Western Blot The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (0.2% BSA, 0.02% sodium azide)
Store at 4°C and avoid from light. For long term storage, dilute the antibody with sterile glycerol and store it at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.