Chicken polyclonal antibody raised against purified Rabbit Actin.
Purified rabbit muscle actin.
Human, Mouse, Rabbit, Rat
Antigen affinity purification
Quality Control Testing:
This antibody was validated by western blot with nitrocellulose membranes (at a concentration of 1 ug/mL) using HRP-labeled goat anti-chicken IgY (1:5000 dilution) as the secondary reagent. Do NOT use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.
Immunocytochemistry Western Blot(1:2500-1:5000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (50% glycerol, 0.02% sodium azide)
Store at -20°C and avoid from light. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.