Rabbit polyclonal antibody against amyloid oligomers.
Synthetic molecular mimic of soluble APP oligomers.
Human, Mouse, Rat
PAB28928 recognizes all types of amyloid oligomers, likely with a peptide backbone epitope that is common in amyloid oligomers but not in native proteins, amyloidogenic monomer or mature amyloid fibrils. A 1:1000 dilution of PAB28928 was sufficient for detection of amyloid oligomers in 10ug of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Protein A affinity purification
Dot blot ELISA Immunofluorescence Immunohistochemistry (1:1000-10000) Immunoprecipitation (1:1000) Western Blot The optimal working dilution should be determined by the end user.
PBS pH7.4, 50% glycerol and 0.09% sodium azide
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Abeta42 fibrils (Lane 1, Lane 3) and prefibrillar oligomers (Lane 2, Lane 4) with APP fibrils polyclonal antibody(Cat # PAB28910; Lane 1, Lane 2) and APP oligomers polyclonal antibody (Cat # PAB28928; Lane 3, Lane 4).
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq