Rabbit polyclonal antibody raised against recombinant Rat Aqp2.
Recombinant protein corresponding to Aqp2 C-terminus.
Human, Mouse, Rat
0.5 ug/mL of PAB28858 was sufficient for detection of Aqp2, ~28.8kDa and sometimes larger glycosylated bands ~35-50kDa, in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Immunofluorescence (1:400) Western Blot (1:1000-4000) The optimal working dilution should be determined by the end user.
PBS pH7.4, 50% glycerol and 0.09% sodium azide
Store at 4°C. Aliquot to avoid repeated freezing and thawing.
Application Data with Unconjugated Antibody
Immunofluorescence staining of Aqp2 in rat kidney with Aqp2 polyclonal antibody (Cat # PAB28856) at 1:200 dilution, DAPI merged.