Rabbit polyclonal antibody raised against recombinant Rat Aqp1.
Recombinant protein corresponding to Aqp1 N-terminus.
Human, Mouse, Rat
0.5 ug/mL of PAB28852 was sufficient for detection of Aqp1, ~28.5kDa and somtimes larger glycosylated bands ~35-50kDa, in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Immunofluorescence (1:200-400) Western Blot (1:1000-4000) The optimal working dilution should be determined by the end user.
PBS pH7.4, 50% glycerol and 0.09% sodium azide
Store at 4°C. Aliquot to avoid repeated freezing and thawing.
Application Data with Unconjugated Antibody
Immunofluorescence staining of Aqp1 in rat kidney with Aqp1 polyclonal antibody (Cat # PAB28838) at 1:200 dilution, DAPI merged.