Scnn1g polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Scnn1g in mCCD (Mouse Cortical Collecting Duct) and FRT cells (mouse kidney) with Scnn1g polyclonal antibody (Cat # PAB28802) at 1:1000 dilution.
Lane 1: mCCD cell lysate
Lane 2: FRT cells expressing tagged Scnn1g
Lane 3: FRT cell lysate
Lane 4: FRT cells expressing tagged Scnn1g
Immunofluorescence
Immunofluorescence staining of Scnn1g in rat kidney with Scnn1g polyclonal antibody (Cat # PAB28802).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Rat Scnn1g.
Immunogen
Recombinant protein corresponding to Scnn1g C-terminus (amino acids 629-650).
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
1 ug/mL of PAB28802 was sufficient for detection of Scnn1g, ~83kDa, in 20ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunofluorescence
Immunohistochemistry
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
PBS, 50% glycerol and 0.09% sodium azide
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Scnn1g in mCCD (Mouse Cortical Collecting Duct) and FRT cells (mouse kidney) with Scnn1g polyclonal antibody (Cat # PAB28802) at 1:1000 dilution.
Lane 1: mCCD cell lysate
Lane 2: FRT cells expressing tagged Scnn1g
Lane 3: FRT cell lysate
Lane 4: FRT cells expressing tagged Scnn1gImmunohistochemistry
Immunofluorescence
Immunofluorescence staining of Scnn1g in rat kidney with Scnn1g polyclonal antibody (Cat # PAB28802). -
Gene Info — Scnn1g
Entrez GeneID
24768Protein Accession#
NP_058742Gene Name
Scnn1g
Gene Alias
ENaC
Gene Description
sodium channel, nonvoltage-gated 1 gamma
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Sodium channel nonvoltage-gated 1 gamma (epithelial)|Sodium channel, nonvoltage-gated 1, gamma (epithelial)|amiloride sensitive sodium channel gamma1 subunit|epithelial sodium channel gamma subunit
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