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Last updated: 2016/12/4

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SLC12A3 polyclonal antibody (ATTO 655)

  • Catalog # : PAB28754
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant Human, Rat SLC12A3.
  • Immunogen:
  • Recombinant protein corresponding to SLC12A3 N-terminus (amino acids 74-95).
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • 1 ug/mL of PAB28754 was sufficient for detection of SLC12A3 ,~160kDa, in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
  • Form:
  • Liquid
  • Conjugation:
  • ATTO 655
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • Immunofluorescence
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • PBS, 50% glycerol and 0.09% sodium azide
  • Storage Instruction:
  • Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Application Data with Unconjugated Antibody
  • Western Blot (Tissue lysate)

    Western blot analysis of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:1000 dilution.
    Lane 1: Cortex
    Lane 2: Cortex X Medulla


    Immunofluorescence

    Immunofluorescence staining of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:200 dilution, DAPI merged.


  • Applications
  • Western Blot
  • Immunofluorescence
  • Application Image
  • Western Blot
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 6559
  • Gene Name:
  • SLC12A3
  • Gene Alias:
  • FLJ96318,NCCT,TSC
  • Gene Description:
  • solute carrier family 12 (sodium/chloride transporters), member 3
  • Gene Summary:
  • This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • Na-Cl symporter,NaCl electroneutral thiazide-sensitive cotransporter,sodium-chloride cotransporter, thiazide-sensitive,solute carrier family 12, member 3,thiazide-sensitive Na-Cl cotransporter
  • Gene Information
  • Gene Name:
  • Slc12a3
  • Gene Alias:
  • NCC,TSC
  • Gene Description:
  • solute carrier family 12 (sodium/chloride transporters), member 3
  • Other Designations:
  • solute carrier family 12, member 3,thiazide-sensitive Na-Cl cotransporter
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