SLC12A3 polyclonal antibody (ATTO 390)
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More Files
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This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody
Western Blot (Tissue lysate)
Western blot analysis of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:1000 dilution.
Lane 1: Cortex
Lane 2: Cortex X Medulla
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody
Immunofluorescence
Immunofluorescence staining of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:200 dilution, DAPI merged.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SLC12A3.
Immunogen
A synthetic peptide corresponding to amino acids 74-95 at N-terminus of rat SLC12A3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
1 ug/mL of PAB28749 was sufficient for detection of SLC12A3 ,~160kDa, in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Form
Liquid
Conjugation
ATTO 390
Purification
Protein A purification
Recommend Usage
Immunocytochemistry/Immunofluorescence
Immunohistochemistry (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated AntibodyWestern Blot (Tissue lysate)
Western blot analysis of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:1000 dilution.
Lane 1: Cortex
Lane 2: Cortex X MedullaImmunofluorescence
Immunofluorescence staining of SLC12A3 in rat kidney with SLC12A3 polyclonal antibody (Cat # PAB28748) at 1:200 dilution, DAPI merged.
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Applications
Western Blot
Immunohistochemistry
Immunofluorescence
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Gene Info — SLC12A3
Entrez GeneID
6559Protein Accession#
NP_000330; NP_062218; P55018; P55017Gene Name
SLC12A3
Gene Alias
FLJ96318, NCCT, TSC
Gene Description
solute carrier family 12 (sodium/chloride transporters), member 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
Na-Cl symporter|NaCl electroneutral thiazide-sensitive cotransporter|sodium-chloride cotransporter, thiazide-sensitive|solute carrier family 12, member 3|thiazide-sensitive Na-Cl cotransporter
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Gene Info — Slc12a3
Entrez GeneID
54300Protein Accession#
NP_000330; NP_062218; P55018; P55017Gene Name
Slc12a3
Gene Alias
NCC, TSC
Gene Description
solute carrier family 12 (sodium/chloride transporters), member 3
Gene Ontology
HyperlinkGene Summary
O
Other Designations
solute carrier family 12, member 3|thiazide-sensitive Na-Cl cotransporter
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Interactome
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Disease
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