Products

Western Blot (Cell lysate)

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla)
with PCMT1 polyclonal antibody (Cat # PAB28722) at 1:100-1:500 dilution.

Immunohistochemistry

Immunohistochemical staining of human stomach with PCMT1 polyclonal antibody (Cat # PAB28722) shows cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Immunofluorescence

Immunofluorescent staining of human cell line A-431 with PCMT1 polyclonal antibody (Cat # PAB28722) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Entrez GeneID

5110

Gene Name

PCMT1

Gene Alias

-

Gene Description

protein-L-isoaspartate (D-aspartate) O-methyltransferase

Omim ID

176851

Gene Ontology

Hyperlink

Gene Summary

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM

Other Designations

L-isoaspartyl/D-aspartyl methyltransferase|OTTHUMP00000017398|OTTHUMP00000017400