MED12 polyclonal antibody
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More Files
- More Functions
Immunohistochemistry
Immunohistochemical staining of human bone marrow with MED12 polyclonal antibody (Cat # PAB28711) show strong nuclear positivity in bone marrow poietic cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with MED12 polyclonal antibody (Cat # PAB28711) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MED12.
Immunogen
Recombinant protein corresponding to amino acids of human MED12.
Sequence
LPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemical staining of human bone marrow with MED12 polyclonal antibody (Cat # PAB28711) show strong nuclear positivity in bone marrow poietic cells.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with MED12 polyclonal antibody (Cat # PAB28711) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli. -
Gene Info — MED12
Entrez GeneID
9968Gene Name
MED12
Gene Alias
CAGH45, FGS1, HOPA, KIAA0192, OKS, OPA1, TNRC11, TRAP230
Gene Description
mediator complex subunit 12
Omim ID
300188Gene Ontology
HyperlinkGene Summary
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq
Other Designations
FG syndrome 1|OPA-containing protein|OTTHUMP00000023491|mediator of RNA polymerase II transcription, subunit 12 homolog|thyroid hormone receptor-associated protein, 230 kDa subunit|trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subuni
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Interactome
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Disease
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