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S100A12 polyclonal antibody

  • Catalog # : PAB28697
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant S100A12.
  • Immunogen:
  • Recombinant protein corresponding to amino acids of human S100A12.
  • Sequence:
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (1:500-1:1000)
    Immunofluorescence (1-4 ug/ml)
    Western Blot (1:100-1:250)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot anyalysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401723) with S100A12 polyclonal antibody (Cat # PAB28697) at 1:100-1:250 dilution.
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of human bone marrow with S100A12 polyclonal antibody (Cat # PAB28697) shows strong nuclear positivity in bone marrow poietic cells at 1:500-1:1000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of human cell line A-431 with S100A12 polyclonal antibody (Cat # PAB28697) at 1-4 ug/mL shows positivity in nucleus but not nucleoli.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6283
  • Gene Name:
  • S100A12
  • Gene Alias:
  • Gene Description:
  • S100 calcium binding protein A12
  • Gene Summary:
  • The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000015332,S100 calcium-binding protein A12,S100 calcium-binding protein A12 (calgranulin C),calgranulin C
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