S100A12 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot anyalysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401723) with S100A12 polyclonal antibody (Cat # PAB28697) at 1:100-1:250 dilution.
Immunohistochemistry
Immunohistochemical staining of human bone marrow with S100A12 polyclonal antibody (Cat # PAB28697) shows strong nuclear positivity in bone marrow poietic cells at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with S100A12 polyclonal antibody (Cat # PAB28697) at 1-4 ug/mL shows positivity in nucleus but not nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant S100A12.
Immunogen
Recombinant protein corresponding to amino acids of human S100A12.
Sequence
KLEEHLEGIVNIFHQYSVRKGHFDTLSKGELKQLLTKELANTIKNIKDKAVIDEIFQGLDANQDEQVDFQEFISLVAIALKAAHYHTHKE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Immunofluorescence (1-4 ug/ml)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot anyalysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401723) with S100A12 polyclonal antibody (Cat # PAB28697) at 1:100-1:250 dilution.Immunohistochemistry
Immunohistochemical staining of human bone marrow with S100A12 polyclonal antibody (Cat # PAB28697) shows strong nuclear positivity in bone marrow poietic cells at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with S100A12 polyclonal antibody (Cat # PAB28697) at 1-4 ug/mL shows positivity in nucleus but not nucleoli. -
Gene Info — S100A12
Entrez GeneID
6283Gene Name
S100A12
Gene Alias
CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6
Gene Description
S100 calcium binding protein A12
Omim ID
603112Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq
Other Designations
OTTHUMP00000015332|S100 calcium-binding protein A12|S100 calcium-binding protein A12 (calgranulin C)|calgranulin C
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Interactome
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Disease
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