Western blot anyalysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401723) with S100A12 polyclonal antibody (Cat # PAB28697) at 1:100-1:250 dilution.
Immunohistochemical staining of human bone marrow with S100A12 polyclonal antibody (Cat # PAB28697) shows strong nuclear positivity in bone marrow poietic cells at 1:500-1:1000 dilution.
Immunofluorescent staining of human cell line A-431 with S100A12 polyclonal antibody (Cat # PAB28697) at 1-4 ug/mL shows positivity in nucleus but not nucleoli.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq
OTTHUMP00000015332,S100 calcium-binding protein A12,S100 calcium-binding protein A12 (calgranulin C),calgranulin C