Western blot anaylsis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with SDHB polyclonal antibody (Cat # PAB28696) at 1:100-1:500 dilution.
Immunohistochemical staining of human liver with SDHB polyclonal antibody (Cat # PAB28696) shows strong cytoplasmic positivity in hepatocytes at 1:500-1:1000 dilution.
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq