CARS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with CARS polyclonal antibody (Cat # PAB28664) at 1:100-1:500 dilution.
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: A-431 with CARS polyclonal antibody (Cat # PAB28664) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with CARS polyclonal antibody (Cat # PAB28664) shows strong cytoplasmic positivity in purkinje cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with CARS polyclonal antibody (Cat # PAB28664) at 1-4 ug/mL shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CARS.
Immunogen
Recombinant protein corresponding to amino acids of human CARS.
Sequence
SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(1:50-1:200)
Immunofluorescence (1-4 ug/ml)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with CARS polyclonal antibody (Cat # PAB28664) at 1:100-1:500 dilution.Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: A-431 with CARS polyclonal antibody (Cat # PAB28664) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with CARS polyclonal antibody (Cat # PAB28664) shows strong cytoplasmic positivity in purkinje cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with CARS polyclonal antibody (Cat # PAB28664) at 1-4 ug/mL shows positivity in cytoplasm. -
Gene Info — CARS
Entrez GeneID
833Gene Name
CARS
Gene Alias
CARS1, CYSRS, MGC:11246
Gene Description
cysteinyl-tRNA synthetase
Omim ID
123859Gene Ontology
HyperlinkGene Summary
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000012605|cysteine tRNA ligase 1, cytoplasmic|cysteine translase|cysteine-tRNA ligase
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Interactome
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Pathway
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Disease
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