SH2D4A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SH2D4A.
Immunogen
Recombinant protein corresponding to amino acids of human SH2D4A.
Sequence
KEEQLPLRAGYQKTSDTIAPWFHGILTLKKANELLLSTGMPGSFLIRVSERIKGYALSYLSEDGCKHFLIDASADAYSFLGVDQLQHATLADLVEYHKEEPITSLGKELLLYPCGQQ
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(1:50-1:200)
Immunofluorescence (1-4 ug/ml)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane 1: Mouse liver tissue lysate, Lane 2: Rat liver tissue lysate with SH2D4A polyclonal antibody (Cat # PAB28656) at 1:100-1:500 dilution.Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: A-431, Lane 4: Human liver with SH2D4A polyclonal antibody (Cat # PAB28656) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with SH2D4A polyclonal antibody (Cat # PAB28656) shows strong cytoplasmic positivity in hepatocytes at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with SH2D4A polyclonal antibody (Cat # PAB28656) at 1-4 ug/mL shows positivity in cytoplasm. -
Gene Info — SH2D4A
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Interactome
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Disease
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