RHOJ polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with RHOJ polyclonal antibody (Cat # PAB28641).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with RHOJ polyclonal antibody (Cat # PAB28641) shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RHOJ.
Immunogen
Recombinant protein corresponding to amino acids of human RHOJ.
Sequence
CFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with RHOJ polyclonal antibody (Cat # PAB28641).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with RHOJ polyclonal antibody (Cat # PAB28641) shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and Leydig cells. -
Gene Info — RHOJ
Entrez GeneID
57381Protein Accession#
Q9H4E5Gene Name
RHOJ
Gene Alias
ARHJ, FLJ14445, MGC34777, RASL7B, TC10B, TCL
Gene Description
ras homolog gene family, member J
Omim ID
607653Gene Ontology
HyperlinkGene Summary
ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.[supplied by OMIM
Other Designations
RAS-like, family 7, member B|TC10-like Rho GTPase
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Interactome
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Disease
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