TOMM22 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TOMM22.
Immunogen
Recombinant protein corresponding to amino acids of human TOMM22.
Sequence
MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQK
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with TOMM22 polyclonal antibody (Cat # PAB28636) at 1:100-1:500 dilution.Western Blot
Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp with TOMM22 polyclonal antibody (Cat # PAB28636) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with TOMM22 polyclonal antibody (Cat # PAB28636) shows strong cytoplasmic positivity in renal tubules at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with TOMM22 polyclonal antibody (Cat # PAB28636) at 1-4 ug/ml shows positivity in mitochondria. -
Gene Info — TOMM22
Entrez GeneID
56993Protein Accession#
Q9NS69Gene Name
TOMM22
Gene Alias
1C9-2, MST065, MSTP065, TOM22
Gene Description
translocase of outer mitochondrial membrane 22 homolog (yeast)
Omim ID
607046Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. [provided by RefSeq
Other Designations
mitochondrial import receptor Tom22
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