SPARC polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with SPARC polyclonal antibody (Cat # PAB28632).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human esophagus with SPARC polyclonal antibody (Cat # PAB28632) shows distinct positivity in fibroblasts.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SPARC.
Immunogen
Recombinant protein corresponding to amino acids of human SPARC.
Sequence
QEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDY
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with SPARC polyclonal antibody (Cat # PAB28632).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human esophagus with SPARC polyclonal antibody (Cat # PAB28632) shows distinct positivity in fibroblasts. -
Gene Info — SPARC
Entrez GeneID
6678Protein Accession#
P09486Gene Name
SPARC
Gene Alias
ON
Gene Description
secreted protein, acidic, cysteine-rich (osteonectin)
Omim ID
182120Gene Ontology
HyperlinkGene Summary
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).[supplied by OMIM
Other Designations
cysteine-rich protein|osteonectin|secreted protein, acidic, cysteine-rich
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Interactome
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Disease
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