CTSS polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CTSS.
Immunogen
Recombinant protein corresponding to amino acids of human CTSS.
Sequence
NGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQYDSKYRAATCSKYTELPYGREDVLKEAVANKGPVSVGVDARHPSFFLYRSGVYYEPSCTQNVNHGVLVVGYGDLNGKEYWLVKNSWGHNFGEEGYIRMARNKGNHCG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with CTSS polyclonal antibody (Cat # PAB28624) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human spleen with CTSS polyclonal antibody (Cat # PAB28624) shows strong cytoplasmic positivity in cells in red pulp at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with CTSS polyclonal antibody (Cat # PAB28624) at 1-4 ug/ml shows positivity in vesicles. -
Gene Info — CTSS
Entrez GeneID
1520Protein Accession#
P25774Gene Name
CTSS
Gene Alias
MGC3886
Gene Description
cathepsin S
Omim ID
116845Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000032942
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Interactome
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Pathway
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Disease
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