POLA1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp, Lane 3: Human cell line A-431 with POLA1 polyclonal antibody (Cat # PAB28620) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with POLA1 polyclonal antibody (Cat # PAB28620) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with POLA1 polyclonal antibody (Cat # PAB28620) at 1-4 ug/ml shows positivity in nucleus but not nucleoli & cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant POLA1.
Immunogen
Recombinant protein corresponding to amino acids of human POLA1.
Sequence
VDLEPMAAKAWDKESEPAEEVKQEADSGKGTVSYLGSFLPDVSCWDIDQEGDSSFSVQEVQVDSSHLPLVKGADEEQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp, Lane 3: Human cell line A-431 with POLA1 polyclonal antibody (Cat # PAB28620) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with POLA1 polyclonal antibody (Cat # PAB28620) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with POLA1 polyclonal antibody (Cat # PAB28620) at 1-4 ug/ml shows positivity in nucleus but not nucleoli & cytoplasm. -
Gene Info — POLA1
Entrez GeneID
5422Protein Accession#
A6NMQ1Gene Name
POLA1
Gene Alias
DKFZp686K1672, POLA, p180
Gene Description
polymerase (DNA directed), alpha 1, catalytic subunit
Omim ID
312040Gene Ontology
HyperlinkGene Summary
alpha 1
Other Designations
OTTHUMP00000023089|polymerase (DNA-directed), alpha|polymerase (DNA-directed), alpha (70kD)
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Interactome
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Pathway
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