Western Blot (1:100-1:250) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500) Immunofluorescence (1-4 ug/ml) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of HEK293T cell lysate using IFI16 polyclonal antibody (Cat # PAB28576). Lane 1: Negative control (vector only) Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skin with IFI16 polyclonal antibody (Cat # PAB28576) shows strong nuclear positivity in epidermal cells. Retrieval method: HIER pH6
Immunofluorescence of U-251 MG cell line with IFI16 polyclonal antibody (Cat # PAB28576) shows positivity in nucleus and nucleoli. Fixation/Permeabilization: PFA/Triton X-104
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. [provided by RefSeq
OTTHUMP00000035192,interferon-gamma induced protein IFI 16,interferon-inducible myeloid differentiation transcriptional activator