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GART polyclonal antibody

  • Catalog # : PAB28573
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant GART.
  • Immunogen:
  • Recombinant protein corresponding to human GART.
  • Sequence:
  • IEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTV
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (Human: 1:100-1:250, Mouse/Rat: 1:100-1:500)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
    Immunofluorescence (1-4 ug/ml)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of lane 1: NIH-3T3 and lane 2: NBT-II cell lysates using GART polyclonal antibody (Cat # PAB28573).
  • Western Blot
  • Western Blot
  • Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: Liver and lane 4: Tonsil using GART polyclonal antibody (Cat # PAB28573).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human duodenum with GART polyclonal antibody (Cat # PAB28573) shows strong cytoplasmic positivity in glandular cells. Retrieval method: HIER pH6
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of U-2 OS cell line with GART polyclonal antibody (Cat # PAB28573) shows positivity in cytoplasm and mitochondria. Fixation/Permeabilization: PFA/Triton X-103
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2618
  • Gene Name:
  • GART
  • Gene Alias:
  • AIRS,GARS,GARTF,MGC47764,PAIS,PGFT,PRGS
  • Gene Description:
  • phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
  • Gene Summary:
  • The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000068280,OTTHUMP00000068281,OTTHUMP00000068282,OTTHUMP00000068337,trifunctional purine biosynthetic protein adenosine-3
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