Western Blot (Human: 1:100-1:250, Mouse/Rat: 1:100-1:500) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200) Immunofluorescence (1-4 ug/ml) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human duodenum with GART polyclonal antibody (Cat # PAB28573) shows strong cytoplasmic positivity in glandular cells. Retrieval method: HIER pH6
Immunofluorescence of U-2 OS cell line with GART polyclonal antibody (Cat # PAB28573) shows positivity in cytoplasm and mitochondria. Fixation/Permeabilization: PFA/Triton X-103
The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
OTTHUMP00000068280,OTTHUMP00000068281,OTTHUMP00000068282,OTTHUMP00000068337,trifunctional purine biosynthetic protein adenosine-3