GART polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against recombinant GART.
Immunogen
Recombinant protein corresponding to human GART.
Sequence
IEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTV
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (Human: 1:100-1:250, Mouse/Rat: 1:100-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of lane 1: NIH-3T3 and lane 2: NBT-II cell lysates using GART polyclonal antibody (Cat # PAB28573).Western Blot
Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: Liver and lane 4: Tonsil using GART polyclonal antibody (Cat # PAB28573).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human duodenum with GART polyclonal antibody (Cat # PAB28573) shows strong cytoplasmic positivity in glandular cells. Retrieval method: HIER pH6Immunofluorescence
Immunofluorescence of U-2 OS cell line with GART polyclonal antibody (Cat # PAB28573) shows positivity in cytoplasm and mitochondria. Fixation/Permeabilization: PFA/Triton X-103 -
Gene Info — GART
Entrez GeneID
2618Protein Accession#
C9JBJ1Gene Name
GART
Gene Alias
AIRS, GARS, GARTF, MGC47764, PAIS, PGFT, PRGS
Gene Description
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Omim ID
138440Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000068280|OTTHUMP00000068281|OTTHUMP00000068282|OTTHUMP00000068337|trifunctional purine biosynthetic protein adenosine-3
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Phosphorylation of seryl-tRNA synthetase by ATM/ATR is essential for hypoxia-induced angiogenesis.
Yi Shi, Ze Liu, Qian Zhang, Ingrid Vallee, Zhongying Mo, Shuji Kishi, Xiang-Lei Yang.
PLoS Biology 2020 Dec; 18(12):e3000991.
Application:WB-Tr, Human, HEK 293 cells.
-
Phosphorylation of seryl-tRNA synthetase by ATM/ATR is essential for hypoxia-induced angiogenesis.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com