Western Blot (Human: 1:100-1:250, Mouse/Rat: 1:100-1:500) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500) Immunofluorescence (1-4 ug/ml) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human hippocampus with PSMD14 polyclonal antibody (Cat # PAB28572) shows strong nuclear positivity in neuronal cells. Retrieval method: HIER pH6
Immunofluorescence of U-2 OS cell line with PSMD14 polyclonal antibody (Cat # PAB28572) shows positivity in nucleus but not nucleoli and vesicles. Fixation/Permeabilization: PFA/Triton X-102