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Last updated: 2017/3/26

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ORAI3 polyclonal antibody

  • Catalog # : PAB28562
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant ORAI3.
  • Immunogen:
  • Recombinant protein corresponding to amino acids of recombinant ORAI3.
  • Sequence:
  • APLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPPRQACGGGGAHGPGWQA
  • Host:
  • Rabbit
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry(1:50-1:200)
    Western Blot(1:100-1:250)
    Immunofluorescence(1-4 ug/ml)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ORAI3 polyclonal antibody (PAB28562) at 1:100-1:250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human testis with ORAI3 polyclonal antibody (Cat # PAB28562) shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts at 1:50-1:200 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of A-431 with ORAI3 polyclonal antibody (Cat # PAB28562) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli & cytoplasm.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • ORAI3
  • Gene Alias:
  • MGC13024,TMEM142C
  • Gene Description:
  • ORAI calcium release-activated calcium modulator 3
  • Other Designations:
  • transmembrane protein 142C
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