OTUB2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with OTUB2 polyclonal antibody (PAB28553).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with OTUB2 polyclonal antibody (Cat # PAB28553) shows distinct nuclear positivity in seminiferus duct cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant OTUB2.
Immunogen
Recombinant protein corresponding to amino acids of recombinant OTUB2.
Sequence
YSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNIL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry(1:200-1:500)
Western Blot(1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with OTUB2 polyclonal antibody (PAB28553).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with OTUB2 polyclonal antibody (Cat # PAB28553) shows distinct nuclear positivity in seminiferus duct cells. -
Gene Info — OTUB2
Entrez GeneID
78990Protein Accession#
Q96DC9Gene Name
OTUB2
Gene Alias
C14orf137, FLJ21916, MGC3102, OTB2, OTU2
Gene Description
OTU domain, ubiquitin aldehyde binding 2
Omim ID
608338Gene Ontology
HyperlinkGene Summary
Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.[supplied by OMIM
Other Designations
ubiquitin-specific protease otubain 2
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