IRF4 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant IRF4.
Immunogen
Recombinant protein corresponding to amino acids of human IRF4.
Sequence
SNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:- 1:)
Immunofluorescence (1-4 ug/ml)
Immunohistochemistry (1:-1:)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with IRF4 polyclonal antibody (Cat # PAB28541) at 1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human IRF4 polyclonal antibody (Cat # PAB28541) shows strong nuclear positivity in lymphoid cells outside reaction centra at 1:250 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251MG with IRF4 polyclonal antibody (Cat # PAB28541) at 1-4 ug/ml shows positivity in nucleus but not nucleoli and cytoplasm. -
Gene Info — IRF4
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Interactomes
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Diseases
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