NFE2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with NFE2 polyclonal antibody (Cat # PAB28536) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human NFE2 polyclonal antibody (Cat # PAB28536) shows strong nuclear positivity in bone marrow poietic cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251MG with NFE2 polyclonal antibody (Cat # PAB28536) at 1-4 ug/ml shows positivity in nucleus but not nucleoli and cytoskeleton (actin filaments).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NFE2.
Immunogen
Recombinant protein corresponding to amino acids of human NFE2.
Sequence
SRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPES
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/ml)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with NFE2 polyclonal antibody (Cat # PAB28536) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human NFE2 polyclonal antibody (Cat # PAB28536) shows strong nuclear positivity in bone marrow poietic cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251MG with NFE2 polyclonal antibody (Cat # PAB28536) at 1-4 ug/ml shows positivity in nucleus but not nucleoli and cytoskeleton (actin filaments). -
Gene Info — NFE2
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Interactome
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