SIX1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with SIX1 polyclonal antibody (Cat # PAB28532) at 1:100-1:500 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with SIX1 polyclonal antibody (Cat # PAB28532) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with SIX1 polyclonal antibody (Cat # PAB28532) shows moderate nuclear positivity in myocytes at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251MG with SIX1 polyclonal antibody (Cat # PAB28532) at 1-4 ug/ml shows positivity in nucleus but not nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SIX1.
Immunogen
Recombinant protein corresponding to amino acids of human SIX1.
Sequence
CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:500)
Immunofluorescence (1-4 ug/ml)
Immunohistochemistry (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with SIX1 polyclonal antibody (Cat # PAB28532) at 1:100-1:500 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with SIX1 polyclonal antibody (Cat # PAB28532) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with SIX1 polyclonal antibody (Cat # PAB28532) shows moderate nuclear positivity in myocytes at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251MG with SIX1 polyclonal antibody (Cat # PAB28532) at 1-4 ug/ml shows positivity in nucleus but not nucleoli. -
Gene Info — SIX1
Entrez GeneID
6495Protein Accession#
Q15475Gene Name
SIX1
Gene Alias
BOS3, DFNA23, TIP39
Gene Description
SIX homeobox 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). [provided by RefSeq
Other Designations
sine oculis homeobox homolog 1
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