Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with ACTN4 polyclonal antibody (Cat # PAB28526) at 1:100-1:500 dilution.
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ACTN4 polyclonal antibody (Cat # PAB28526) at 1:100-1:250 dilution.
Immunohistochemical staining of human liver with ACTN4 polyclonal antibody (Cat # PAB28526) shows strong cytoplasmic and membranous positivity in lymphoid tissue in bile duct cells at 1:1000-1:2500 dilution.
Immunofluorescent staining of human cell line A-431 with ACTN4 polyclonal antibody (Cat # PAB28526) at 1-4 ug/ml shows positivity in nucleus.
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq