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POLG polyclonal antibody

  • Catalog # : PAB28515
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of POLG
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 1120-1148 at C-terminal region at human POLG.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Flow Cytometry (1:10-50)
    Immunohistochemistry (1:10-50)
    Western Blot (1:1000)
    Immunofluorescence (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of POLG polyclonal antibody (Cat # PAB28515) in mouse cerebellum tissue lysates (35ug/lane). POLG (arrow) was detected using the purified polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skeletal muscle with POLG polyclonal antibody (Cat # PAB28515) followed by peroxidase conjugation of the secondary antibody and DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence confocal analysis of POLG using POLG polyclonal antibody (Cat # PAB28515) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).
    DAPI was used to stain the cell nuclear (blue).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of CEM cells using POLG polyclonal antibody (Cat # PAB28515) (right histogram) compared to a negative control cell (left histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5428
  • Gene Name:
  • POLG
  • Gene Alias:
  • FLJ27114,MDP1,PEO,POLG1,POLGA,SANDO,SCAE
  • Gene Description:
  • polymerase (DNA directed), gamma
  • Gene Summary:
  • Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • DNA polymerase gamma,DNA polymerase subunit gamma-1,PolG, catalytic subunit,PolG-alpha,mitochondrial DNA polymerase catalytic subunit
  • Gene Pathway
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