Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skeletal muscle with POLG polyclonal antibody (Cat # PAB28515) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescence confocal analysis of POLG using POLG polyclonal antibody (Cat # PAB28515) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of CEM cells using POLG polyclonal antibody (Cat # PAB28515) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
DNA polymerase gamma,DNA polymerase subunit gamma-1,PolG, catalytic subunit,PolG-alpha,mitochondrial DNA polymerase catalytic subunit