RBM22 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RBM22.
Immunogen
Recombinant protein corresponding to amino acids of human RBM22
Sequence
NTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKATSTSDMLLKL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry(1:20-1:50)
Western Blot(1:100-1:250)
Immunofluorescence(1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with RBM22 polyclonal antibody at 1:100-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with RBM22 polyclonal antibody (Cat # PAB28467) shows strong nuclear positivity in neuronal cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251MG with RBM22 polyclonal antibody (Cat # PAB28467) at 1-4 ug/ml shows positivity in nuclei but not nucleoli. -
Gene Info — RBM22
Entrez GeneID
55696Protein Accession#
Q9NW64Gene Name
RBM22
Gene Alias
FLJ10290, ZC3H16, fSAP47
Gene Description
RNA binding motif protein 22
Gene Ontology
HyperlinkGene Summary
This gene encodes an RNA binding protein. The encoded protein may play a role in cell division and may be involved in pre-mRNA splicing. Related pseudogenes exist on chromosomes 6, 7, 9, 13, 16, 18, and X. [provided by RefSeq
Other Designations
functional spliceosome-associated protein 47
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