ELK3 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ELK3 polyclonal antibody (Cat # PAB28457) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human appendix with ELK3 polyclonal antibody (Cat # PAB28457) shows moderate nuclear positivity in lymphoid tissue at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of A-431 with ELK3 polyclonal antibody (Cat # PAB28457) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ELK3.
Immunogen
Recombinant protein corresponding to amino acids of recombinant ELK3.
Sequence
KNIIKKVIGQKFVYKFVSFPEILKMDPHAVEISRESLLLQDSDCKASPEGREAHKHGLAALRSTSRNEYIHSGLYSSFTINSLQNPPDAFKAIKTEKLEEPPEDSPPVEEVRTVIRFVTNKTDKHVTR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry(1:500-1:1000)
Western Blot(1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ELK3 polyclonal antibody (Cat # PAB28457) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human appendix with ELK3 polyclonal antibody (Cat # PAB28457) shows moderate nuclear positivity in lymphoid tissue at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of A-431 with ELK3 polyclonal antibody (Cat # PAB28457) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli. -
Gene Info — ELK3
Entrez GeneID
2004Protein Accession#
P41970Gene Name
ELK3
Gene Alias
ERP, NET, SAP2
Gene Description
ELK3, ETS-domain protein (SRF accessory protein 2)
Omim ID
600247Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq
Other Designations
ELK3 protein|ETS-domain protein|SRF accessory protein 2
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Interactome
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Disease
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