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CYB5R3 polyclonal antibody

  • Catalog # : PAB28452
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant CYB5R3.
  • Immunogen:
  • Recombinant protein corresponding to amino acids of recombinant CYB5R3.
  • Sequence:
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry(1:1000-1:2500)
    Western Blot(1:100-1:250)
    Immunofluorescence (1-4 ug/ml)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with CYB5R3 polyclonal antibody (Cat # PAB28452) at 1:100-1:250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human testis with CYB5R3 polyclonal antibody (Cat # PAB28452) shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells at 1:1000-1:2500 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of U373 MG with CYB5R3 polyclonal antibody (Cat # PAB28452) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Entrez GeneID:
  • 1727
  • Gene Name:
  • CYB5R3
  • Gene Alias:
  • B5R,DIA1
  • Gene Description:
  • cytochrome b5 reductase 3
  • Gene Summary:
  • This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq
  • Other Designations:
  • NADH-cytochrome b5 reductase,OTTHUMP00000028761,cytochrome b5 reductase,diaphorase (NADH) (cytochrome b-5 reductase)
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