Immunohistochemical staining of human testis with CYB5R3 polyclonal antibody (Cat # PAB28452) shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells at 1:1000-1:2500 dilution.
Immunofluorescent staining of U373 MG with CYB5R3 polyclonal antibody (Cat # PAB28452) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum.
This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq