NAE1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of NAE1 polyclonal antibody (Cat # PAB2845) in mouse brain tissue lysates (35 ug/lane). NAE1 (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of NAE1 (arrow) using rabbit NAE1 polyclonal antibody (Cat # PAB2845). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NAE1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NAE1 polyclonal antibody (Cat # PAB2845) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NAE1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NAE1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
Western blot analysis of NAE1 polyclonal antibody (Cat # PAB2845) in mouse brain tissue lysates (35 ug/lane). NAE1 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of NAE1 (arrow) using rabbit NAE1 polyclonal antibody (Cat # PAB2845). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NAE1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NAE1 polyclonal antibody (Cat # PAB2845) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — NAE1
Entrez GeneID
8883Protein Accession#
Q13564;NP_003896Gene Name
NAE1
Gene Alias
A-116A10.1, APPBP1, HPP1, ula-1
Gene Description
NEDD8 activating enzyme E1 subunit 1
Omim ID
603385Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
NEDD8-activating enzyme E1 subunit|amyloid beta precursor protein binding protein 1, 59kDa|amyloid beta precursor protein-binding protein 1, 59kD|amyloid protein-binding protein 1|protooncogene protein 1
-
Interactome
-
Publication Reference
-
APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
Chen Y, Liu W, McPhie DL, Hassinger L, Neve RL.
Journal of Cellular Biology 2003 Oct; 163(1):27.
-
ASPP2 inhibits APP-BP1-mediated NEDD8 conjugation to cullin-1 and decreases APP-BP1-induced cell proliferation and neuronal apoptosis.
Chen Y, Liu W, Naumovski L, Neve RL.
Journal of Neurochemistry 2003 May; 85(3):801.
Application：WB-Tr, Human, HeLa, HEK 293 cells.
-
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA.
Nature 2003 Mar; 422(6929):330.
-
APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com